The polystyrene composites, received by application polymeric solution (polystyrene) on teflon base sheet with the following samples drying at 293 K to constant mass were studied. The hydroxiapatite particles immobilization in polysterene matrix was made by mechanical dispersion. The polimeric’s systames hydroxiapatite concentration were 0,03; 0,1; 0,5 mac.%. The authors determined sorption and structural hydroxiapatite’s characteristics such as specific surface area and pore volume. The authors studied the influence of hydroxiapatite concentration in composites on lipid free radical processes and antioxidant activity of confluent blood serum and confluent serum after contact with composite, including hydroxiapatite in vitro. It was noted that polymeric composites with 0,1 and 0,5 mac% hydroxiapatite concentration increase the hemiluminescence parameters based on hemiluminescence analysis: fast flashes Imax and lightsum S. These data argue for free radical processes activation, so curve slope ratio increased tga and parameters A and Z decreased. It’s reflects the antioxidant activity increasing. The authors performed the investigation of malonic dialdehyde content and total blood serum antioxidant activity by spectrophotometry. The antioxidant activity increasing after composites impact, including 0.5mac% hydroxiapatite in confluent blood serum was determined. These data argue for composites’ antioxidant effects in comparison with control blood serum.
hydroxiapatite, lipid free radical process, antioxidant activity, hemiluminescence.
1. Alekseeva O.V., Bagrovskaya N.A., Sitnikova O.G., Nazarov S.B. Issledovanie antioksidantnoy aktivnosti nanokompozitov na osnove polistirola in vitro. Zhidkie kristally i ikh prakticheskoe is-pol´shchovanie. 2011.№3. S. 90-96.
2. Zakharov N.A., Skibinskiy K.V., Bayrikov I.M., Mizina P.G., Kuz´mina V.E., Ermak I.M., Chalykh A.E., Kalinnikov V.T. Cintez i issledovanie organomineral´nogo nanokompozita gidroksiapa-tit kal´tsiya/karraginan. Kondensirovannye sredy i mezhfaznye granitsy. 2007. T. 9. № 2. S. 112-124.
3. Pisareva E.V., Vlasov M.Yu., Orlova E.V. Vliya-nie allogennogo gidroksiapatita na aktivnost´ katalazy, uroven´ dienovykh i malonovogo dial´degida u krys. Vestnik Samarskogo gosudarstvennogo universiteta. Estestvennonauchnaya seriya. 2012. № 9(100). S. 217-225.
4. Promyslov M.Sh., Demchuk M.L. Modifikatsiya metoda opredeleniya antioksidantnoy aktivnosti syvorotki krovi. Voprosy meditsinskoy khimii. 1990. № 4. S. 90-92.
5. Rebrova O.Yu. Statisticheskiy analiz medi-tsinskikh dannykh. Primenenie paketa prikladnykh programm STATISTICA. M.: MediaSfera, 2002. 312 s.
6. Rozhinskaya L. Ya. Sistemnyy osteoporoz. M.: Mokeev, 2000. S. 29-51.
7. Sitnikova O.G., Nazarov S.B., Dyuzhev Zh.A., Klycheva M.M., Popova I.G., Alekseeva O.V., Agafo-nov A.V. Issledovanie vliyaniya razlichnykh vidov nanorazmernogo dioksida kremniya na razvitie ok-sidantnogo stressa i antioksidantnuyu aktivnost´ in vitro. Vestnik novykh meditsinskikh tekhnologiy. 2013. № 3. S. 17-21.
8. Ishihara M. Studies on lipoperoxide of normal pregnant women and patient toxemia of pregnancy. Clin. Chim. Acta. 1978. Vol. 84. P. 1-9.
9. Effect of calcium carbonate on clinical com-pliance of apatitic calcium phosphate bone cement / Khairoun I. [et al.]. J Biomed Mater Res. 1997. № 38. P. 356-360.
10. Chapman M.W., Bucholz R., Cornell C. Trea-ment of acute fractures with a collagen-calcium phos-phate graft material. A randomized clinical trial. J. Bone Joint. Surg. Am. 1997. № 4. P. 495-502.
11. Oxidative stress and apoptosis induced by hydroxyapatite nanoparticles in C6 cells / Xu J. [et al.]. J. Biomed Mater Res A. 2012. № 3. R. 738-745.
