Russian Federation
Russian Federation
Russian Federation
Russian Federation
Irkutsk Scientific Center SB RAS (otdel mediko-biologicheskih issledovaniy i tehnologiy, zaveduyuschiy)
Irkutsk, Irkutsk region, Russian Federation
Purpose of the study. To rate prognostic properties of changes in mitochondrial DNA concentration in the blood plasma of patients with chronic cerebral ischemia and ischemic heart disease in relation to the disease and the effectiveness of the therapy. Materials and methods. The study involved patients suffering from coronary heart disease (CHD) and chronic cerebral ischemia (CCI) with stable and unstable atherosclerotic plaques, who have signed informed consent to the data processing within the framework of scientific research. The patients were admitted to the hospital for examination and treatment of CHD and CCI in Cardiology and Neurology Unit of the Hospital of ISC SB RAS. The subjects underwent laboratory and instrumental examination and analysis of the level of free circulating serum mitochondrial DNA by real-time PCR (copies/ml). The examination results considered as satisfactory were compared with the mtDNA levels before and after the treatment. Results. The average value of the mtDNA levels before and after the treatment in patients of neurological and cardiological profile were significantly different: 1 093 686 copies/ml vs 418 046 copies/ml, respectively (p = 0.02). Unlike women, men mtDNA levels statistically significantly (p = 0.03) decreased after the treatment. We revealed statistically significant differences in mtDNA level indicators before and after the treatment, depending on the definition of the series (p = 0.0010) for rank test Kruskal – Wallis test. The results of the proposed research will help to identify prognostic factors of destabilization of cell damage and plaques in endothelial dysfunction, atherosclerosis and its complications, to conduct clinical test of the method for predicting and diagnostics of cellular damage in chronic ischemia on a background of atherosclerosis.
clinical research, atherosclerosis, freely circulating mitochondrial DNA, coronary heart disease, c hronic c erebral isc hemia
В настоящее время наблюдается значительный прогресс в изучении и понимании сердечно-сосудистой патологии, тем не менее, остаётся ряд нерешённых вопросов в диагностике и прогнозе течения патологических процессов, что важно при выборе тактики лечения и реабилитации пациентов. Очевидно, что необходима разработка новых подходов к диагностике и выявлению факторов риска для данной категории больных. Разработка новых способов диагностики и прогнозирования органных и клеточных повреждений, выявление ключевых факторов рисков нестабильности течения эндотелиальной дисфункции и атеросклероза направлены на решение важнейшей проблемы современной медицины – профилактику, сохранение и укрепление здоровья населения.
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