The purpose of this work is the study of microcirculation state at different local effects of reactive oxygen species on animal tissues. The experiment was performed on 60 healthy white Wistar rats. Control group (without any manipulations) included 20 Wistar rats. Also 4 main groups were formed (10 rats in each). Rats of the first group (n=10) were intracutaneously injected with 1 ml of oxygen (in the back), a local darsonvalization during 4 min. was carried out in animals of the second group (n=10). In third group (n=10) the injection of 1 ml of oxygen-ozone mixture was made, in the fourth group (n=10) - darsonvalization during 4 min. after a preliminary introduction of oxygen (1 ml). Microcirculation parameters were tested by laser Doppler flowmetry with LAKK-02 device. It was defined, that local effects of reactive oxygen species include microcirculation changes, depending from specialties of exposed factor.
microcirculation, ozone, oxygen, local administration, darsonvalization
1. Andreeva N.N., Peretyagin S.P. Korrigiruyushchee deystvie ekstrakorporal´nogo ozonirovaniya na spektr zhirnykh kislot plazmy i miokarda v postreanimatsionnom periode. Ozon v biologii i meditsine: Tez. dokl. I Vseros. nauch.-prakt. konf. N.Novgorod, 1992. S. 21-22.
2. Vanin A.F. Oksid azota v biomeditsinskikh issledovaniyakh. Vestnik RAMN. 2000. №4. S. 3-5.
3. Deystvie dinitrozil´nogo kompleksa zheleza na metabolizm i kletochnye membrany ishemizirovannogo serdtsa krysy / Vanin A.F., Pisarenko O.I., Studneva I.M. [i dr.].. Kardiologiya. 2009. №12. C. 43-49.
4. Dubinina E.E. Rol´ aktivnykh form kisloroda v kachestve signal´nykh molekul v metabolizme tkaney pri sostoyaniyakh okislitel´nogo stressa. Voprosy meditsinskoy khimii. 2001. T. 47, №6. C. 561- 581.
5. Korol´kova T.N., Poliychuk T.P. Izuchenie pokazateley oksidativnogo stressa pri kislorodno-ozonovoy terapii lokal´nykh zhirovykh otlozheniy. Klinicheskaya dermatologiya i venerologiya. 2009. №2. C. 37-42.
6. Ozonoterapiya kak metod korrektsii mikrotsirkulyatornykh narusheniy kozhi u bol´nykh s ekzemoy / Kosheleva I.V. , Ivanov O.L., Kulikov A.G. [i dr.]. Rossiyskiy zhurnal kozhnykh i venericheskikh bolezney. 2003. №2. C. 35-45.
7. Lipovetskiy B.M. Klinicheskaya lipidologiya. Sankt-Peterburg: Nauka, 2000.
8. Eksperimental´naya otsenka vliyaniya lekarstvennykh kompozitsiy na sostoyanie mikrotsirkulyatsii v rannem posleozhogovom periode / Martusevich A.K., Larionova K.D., Peretyagin S.P. [i dr.]. Fundamental´nye issledovaniya. 2013. №3. C. 332-336.
9. Martusevich A.K., Peretyagin S.P. Molekulyarnaya stereotipiya v realizatsii effekta nekotorykh lechebnykh fiziko-khimicheskikh faktorov: rol´ NO. Vestnik Nizhegorodskogo universiteta im. N.I. Lobachevskogo. 2012. №2, Ch. 3. C. 205-210
10. Mikhaylichenko L.A., Tikhomirova I.A. Pokazateli mikrotsirkulyatsii i otsenka mekhanizmov regulyatsii tonusa sosudov kozhi krys v usloviyakh modifikatsii reologicheskikh svoystv krovi. Regionarnoe krovoobrashchenie i mikrotsirkulyatsiya. 2012. №1. C. 73-81.
11. Peretyagin S.P. O mnogofaktornom mekhanizme lechebnogo deystviya ozona. Nizhegorodskiy meditsinskiy zhurnal. 2003. Pril. «Ozonoterapiya». C. 6-7.
12. Peretyagin S.P. , Martusevich A.K., Peretyagin P.V., Martusevich A.A. Issledovanie reaktsii sistemy mikrotsirkulyatsii organizma krysy na ingalyatsionnuyu ozonoterapiyu. Vestnik fizioterapii i kurortologii. 2012. T. 17, №5. C. 32-34.
13. Primenenie ozona kak sredstva detoksikatsii v rannem periode ozhogovoy bolezni / Peretyagin S.P., Struchkov A.A. , Martusevich A.K. [i dr.]. Skoraya meditsinskaya pomoshch´. 2011. T. 12, №3. C. 39-43.
14. Reutov V.P., Sorokina E.G., Okhotin V.E., Kositsyn N.S. Tsiklicheskie prevrashcheniya oksida azota v organizme mlekopitayushchikh. M.: Nauka, 1997. 156 s.
15.
16.
17.
18.
19.
20.
21.
22.
23.
