Detailed information about qualitative and quantitative composition of individual components of the group sapropel from the lake Glubokoe (Tatarstan), as well as on the biological activity of different sapropel preparations were obtained by means of the methods Fourier spectroscopy, UV/Vis, NMR spectroscopy, gas chromatography-mass spectrometry, preparative Thin-layer chro-matography (TLC) with witnesses, elemental and functional analysis. Sequential scheme of extraction, acid-alkaline hydrolysis, preparative TLC source of sapropel and individual components of organic matter was developed. Amino acids, sugars, carboxylic acids, alcohols, ketones, flavonida, n-, ISO - and cycloalkanes, vitamins, derivatives of phenol, naftalan, chlorophyll, carotenoids, quinones, anthocyanins, metalloporphyrins were identified. Most of them have close genetic relationship with the source material, which is involved in sapropel formation, having a high biological activity. Biological testing of different sapropel preparations using bacteria St. Aureus, E. Coli, C. Diphythriac gravis, the fungi of the Candida albicans type were made. Significant bactericidal effect of sapropel preparations was established and it is comparable to those for synthetic antibiotics type hydro-cortisone, prednisolone.
sapropel, phytoplankton, humic substances, chromatography, spectroscopy, gas chromatography-mass spectrometry.
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