Voronezh, Voronezh, Russian Federation
employee
Voronezh, Voronezh, Russian Federation
employee
All-Russian research institute of forest genetics, selection and biotechnology (Director)
employee
Voronezh, Voronezh, Russian Federation
employee
Voronezh, Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
employee
Voronezh, Voronezh, Russian Federation
The article discusses the use of microsatellite (SSR) markers for the estimation of genetic resources of birch. It is shown that DNA analysis using 12 microsatellites is most suitable for intraspecific differentiation of selectively valua-ble genotypes of this tree species. The genetic polymorphism in the studied birch genotypes was determined from the loci examined by SSR. Multilocus genetic passports were prepared for 31 samples of birch, which allowed (based on the presence (1) / absence (0) of 36 alleles) to identify the investigated samples.
birch, Betula, genotyping, microsatellites, SSR, polymorphism, PCR, passportization.
Введение. В настоящее время методы молекулярной генетики всё шире применяются в лесном хозяйстве для оценки и мониторинга состояния лесных генетических ресурсов, управления процессами лесовосстановления, фитосанитарного мониторинга лесных насаждений и питомников, получения селекционного посадочного материала. Одной из основных задач лесного хозяйства является лесовосстановление и лесоразведение. Быстрое и качественное лесовосстановление возможно только при выполнении ряда условий, таких как использование на плантациях улучшенного посадочного материала и его надежная идентификация, в том числе и с применением молекулярно-генетических методов.
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