COMPARATIVE CHARACTERISTICS OF IMMUNOLOGICAL RECONSTRUCTION OF WHITE MICE IMMUNIZED BY CELL WALL OF DIFFERENT SUBSPECIES OF FRANCISELLA TULARENSIS
Rubrics: EXPERIMENTAL RESEARCH
Authors:
1.
Irkutsk Antiplague Research Institute of Siberia and Far East
(Laboratory of Pathophysiology, Head)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
2.
Irkutsk Antiplague Research Institute of Siberia and Far East
(Laboratory of Pathophysiology, Research Officer)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
3.
Irkutsk Antiplague Research Institute of Siberia and Far East
(Department of Plague Microbiology, Senior Research Officer)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
4.
Irkutsk Antiplague Research Institute
(Biochemical Department, Junior Research Officer)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
5.
Irkutsk Antiplague Research Institute
(Laboratory of Experimental Animals, Head)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
6.
Irkutsk Antiplague Research Institute of Siberia and Far East
(Laboratory of Pathophysiology, Research Assistant)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
7.
Irkutsk Antiplague Research Institute of Siberia and Far East awarded with Order of the Red Banner
(Director)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
Type:
Article
Pages:
from 101
to 106
Status:
Published
Received:
02.11.2017
Accepted:
02.11.2017
Published:
02.11.2017
Subject area:
UDK 61 Медицина. Охрана здоровья
Language:
Russian
Keywords:
Francisella tularensis, apudocity, kletochnye stenki, morfometriya
Abstract (English):
V nastoyaschee vremya aktual'noy problemoy yavlyaetsya razrabotka effektivnyh vakcin novogo pokoleniya, v chastnosti v otnoshenii vozbuditelya tulyaremii, chto obuslavlivaet poisk antigennyh determinant s vysokoy immunogennoy aktivnost'yu. Ryadom avtorov pokazano, chto belki naruzhnoy membrany F. tula- rensis obladayut immunologicheskoy aktivnost'yu. Dannyy fakt posluzhil osnovaniem dlya polucheniya i vsestoronnego izucheniya kletochnyh obolochek tulyaremiynogo mikroba kak perspektivnogo komponenta pri konstruirovanii vakcin. Provedena ocenka morfologicheskih izmeneniy v immunokompetentnyh organah eksperimental'nyh zhivotnyh, immunizirovannyh kletochnymi stenkami Francisella tularensis raznyh podvidov. Dlya vydeleniya kletochnyh stenok v kachestve shtammov-producentov ispol'zovali F. tularensis subsp. mediaasiatica A-61, F. tularensis subsp. tularensis novicida V-399 A-Cole, F. tularensis subsp. holarctica 306 i vakcinnogo shtamma F. tularensis subsp. holarctica 15 NIIEG. Pokazano, chto kletochnye stenki F. tularensis nezavisimo ot podvidovoy prinadlezhnosti shtamma- producenta okazyvayut stimuliruyuschee deystvie na vyrabotku antiteloobrazuyuschih kletok, a takzhe na kletochnuyu proliferaciyu v T-zavisimyh zonah limfaticheskih uzlov i selezenke. Ustanovleno, chto vvedenie etih antigennyh preparatov privodit k uvelicheniyu parakortikal'noy zony, korkovogo veschestva limfaticheskih uzlov i uvelicheniyu kolichestva plazmaticheskih kletok i malyh limfocitov. Ustanovleno, chto kletochnye stenki F. tularensis raznyh podvidov ne vyzyvayut stressovoy reakcii organizma eksperimental'nyh zhivotnyh, poskol'ku vo vse sroki issledovaniya v nadpochechnikah eksperimental'nyh zhivotnyh pokazateli soderzhaniya v opytnyh obrazcah argirofil'nyh i argentaffinnyh kletok sootvetstvovali znacheniyam v kontrole. Na osnovanii poluchennyh dannyh suschestvuet neobhodimost' dal'neyshego detal'nogo issledovaniya immunogennyh svoystv kletochnyh obolochek F. tularensis subsp. holarctica 306, F. tularensis subsp. mediasiatica A-61 i F. tularensis subsp. tularensis B-399 A-Cole v kachestve perspektivnyh komponentov pri konstruirovanii vakcin protiv tulyaremii.
V nastoyaschee vremya aktual'noy problemoy yavlyaetsya razrabotka effektivnyh vakcin novogo pokoleniya, v chastnosti v otnoshenii vozbuditelya tulyaremii, chto obuslavlivaet poisk antigennyh determinant s vysokoy immunogennoy aktivnost'yu. Ryadom avtorov pokazano, chto belki naruzhnoy membrany F. tula- rensis obladayut immunologicheskoy aktivnost'yu. Dannyy fakt posluzhil osnovaniem dlya polucheniya i vsestoronnego izucheniya kletochnyh obolochek tulyaremiynogo mikroba kak perspektivnogo komponenta pri konstruirovanii vakcin. Provedena ocenka morfologicheskih izmeneniy v immunokompetentnyh organah eksperimental'nyh zhivotnyh, immunizirovannyh kletochnymi stenkami Francisella tularensis raznyh podvidov. Dlya vydeleniya kletochnyh stenok v kachestve shtammov-producentov ispol'zovali F. tularensis subsp. mediaasiatica A-61, F. tularensis subsp. tularensis novicida V-399 A-Cole, F. tularensis subsp. holarctica 306 i vakcinnogo shtamma F. tularensis subsp. holarctica 15 NIIEG. Pokazano, chto kletochnye stenki F. tularensis nezavisimo ot podvidovoy prinadlezhnosti shtamma- producenta okazyvayut stimuliruyuschee deystvie na vyrabotku antiteloobrazuyuschih kletok, a takzhe na kletochnuyu proliferaciyu v T-zavisimyh zonah limfaticheskih uzlov i selezenke. Ustanovleno, chto vvedenie etih antigennyh preparatov privodit k uvelicheniyu parakortikal'noy zony, korkovogo veschestva limfaticheskih uzlov i uvelicheniyu kolichestva plazmaticheskih kletok i malyh limfocitov. Ustanovleno, chto kletochnye stenki F. tularensis raznyh podvidov ne vyzyvayut stressovoy reakcii organizma eksperimental'nyh zhivotnyh, poskol'ku vo vse sroki issledovaniya v nadpochechnikah eksperimental'nyh zhivotnyh pokazateli soderzhaniya v opytnyh obrazcah argirofil'nyh i argentaffinnyh kletok sootvetstvovali znacheniyam v kontrole. Na osnovanii poluchennyh dannyh suschestvuet neobhodimost' dal'neyshego detal'nogo issledovaniya immunogennyh svoystv kletochnyh obolochek F. tularensis subsp. holarctica 306, F. tularensis subsp. mediasiatica A-61 i F. tularensis subsp. tularensis B-399 A-Cole v kachestve perspektivnyh komponentov pri konstruirovanii vakcin protiv tulyaremii.
Keywords:
Francisella tularensis, apudocity, kletochnye stenki, morfometriya
Francisella tularensis, apudocity, kletochnye stenki, morfometriya
1. Spislit immunologicheskaya perestroyka
