CHARACTERISTICS OF GENETIC AND PHENOTYPIC PROPERTIES OF TICK-BORNE ENCEPHALITIS VIRUS STRAINS ISOLATED FROM VARIOUS SOURCE ON THE TERRITORY OF EASTERN SIBERIA
Rubrics: MICROBIOLOGY AND VIROLOGY
Authors:
1.
Centre of Hygiene and Epidemiology in the Irkutsk Region
(Biologist at the Virology Laboratory)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
2.
Scientific Center for Human Reproduction and Family Health Problems
(Laboratory of Molecular Epidemiology and Genetic Testing, Head)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
3.
Scientific Center for Human Reproduction and Family Health Problems
(Laboratory of Molecular Epidemiology and Genetic Testing, Research Officer)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
4.
Scientific Center for Human Reproduction and Family Health Problems
(Laboratory of Molecular Epidemiology and Genetic Testing, Junior Research Officer)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
5.
Irkutsk State Agrarian University named after A.A. Ezhevsky
(Department of the Technology of Production and Processing of Agricultural Products and Veterinary-Sanitary Evaluation, Professor)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
6.
Institute of Chemical Biology and Fundamental Medicine
(Junior Research Officer)
Novosibirsk, Novosibirsk, Russian Federation
Novosibirsk, Novosibirsk, Russian Federation
7.
Irkutsk State Medical University
(Senior Research Officer)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
8.
Scientific Center for Human Reproduction and Family Health Problems
(Laboratory of Molecular Epidemiology and Genetic Testing, Research Officer)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
9.
Scientific Centre for Family Health and Human Reproduction Problems
(Laboratory of Molecular Epidemiology and Genetic Testing, Junior Research Officer)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
10.
Scientific Centre for Family Health and Human Reproduction Problems
(Laboratory of Molecular Epidemiology and Genetic Testing, Clinical Research Assistant)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
11.
Irkutsk State Medical University
(Department of Microbiology, Virology and Immunology with the Course of Clinical Laboratory Diagnostics, Head)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
Type:
Article
Pages:
from 76
to 82
Status:
Published
Received:
19.10.2017
Accepted:
22.11.2017
Published:
22.11.2017
Subject area:
UDK 61 Медицина. Охрана здоровья
Language:
Russian
Keywords:
virus kleschevogo encefalita, genotip, geneticheskie svoystva, fenotipicheskie svoystva, geneticheskie markery, istochnik izolyacii, Vostochnaya Sibir'
Abstract (English):
V svyazi s tem, chto osnovu formirovaniya populyacii virusa kleschevogo encefalita (VKE) sostavlyayut iksodovye kleschi i ih prokormiteli – pozvonochnye zhivotnye, predstavlyalo interes poluchenie geneticheskoy harakteristiki shtammov, vydelennyh iz razlichnyh istochnikov na territorii Vostochnoy Sibiri. V hode provedennogo issledovaniya ustanovleno, chto cirkulyaciya VKE genotipov 1, 2, 3, 5 i «politipovyh» shtammov na territorii Vostochnoy Sibiri podderzhivaetsya kak osnovnymi perenoschikami (kleschami I. persulcatus), tak i ih prokormitelyami (melkimi i krupnymi mlekopitayuschimi, pticami). Raspredelenie genotipov v gruppah shtammov ot iksodovyh kleschey i pozvonochnyh zhivotnyh razlichalos'. Shtammy VKE genotipov 1 i 2 dostoverno chasche vydelyalis' ot gryzunov, genotipa 3 - ot iksodovyh kleschey (p˂0,05). V gruppe shtammov, izolirovannyh ot iksodovyh kleschey preobladali izolyaty genotipa 3 (85,5%), iz kotoryh 29,6% sostavili shtammy subgenotipa «Vasil'chenko», 25,6% - subgenotipa «Zausaev». Genotipy 1 i 2 byli predstavleny edinichnymi izolyatami (3,3% i 1,3% sootvetstvenno). Sredi shtammov, izolirovannyh ot teplokrovnyh hozyaev, dolya genotipa 1 sostavila 35,3%, genotipa 2 –11,8%, genotipa 3 - 35,3%. Bolee poloviny shtammov genotipa 3 (58,3%) byli otneseny k subgenotipu «Vasil'chenko», shtammov subgenotipa «Zausaev» ne vyyavleno. Poluchennye dannye pozvolyayut predpolozhit', chto iksodovye kleschi yavlyayutsya amplifikatorami VKE genotipa 3, a teplokrovnye zhivotnye – genotipa 1. Vyyavleny opredelennye razlichiya fenotipicheskih priznakov shtammov, izolirovannyh ot kleschey i teplokrovnyh zhivotnyh. Ustanovleno, chto shtammy, izolirovannye ot kleschey, bolee odnorodny po svoey antigennoy harakteristike, menee geterogenny po S-priznaku, chem shtammy, vydelennye ot teplokrovnyh istochnikov. Shtammy, vydelennye ot teplokrovnyh proyavlyayut neskol'ko bol'shuyu ustoychivost' k progrevaniyu pri odnovremennom snizhenii reprodukcii virusa pri temperature 42°S, obladayut bol'shey virulentnost'yu po sravneniyu so shtammami, vydelennymi ot kleschey.
V svyazi s tem, chto osnovu formirovaniya populyacii virusa kleschevogo encefalita (VKE) sostavlyayut iksodovye kleschi i ih prokormiteli – pozvonochnye zhivotnye, predstavlyalo interes poluchenie geneticheskoy harakteristiki shtammov, vydelennyh iz razlichnyh istochnikov na territorii Vostochnoy Sibiri. V hode provedennogo issledovaniya ustanovleno, chto cirkulyaciya VKE genotipov 1, 2, 3, 5 i «politipovyh» shtammov na territorii Vostochnoy Sibiri podderzhivaetsya kak osnovnymi perenoschikami (kleschami I. persulcatus), tak i ih prokormitelyami (melkimi i krupnymi mlekopitayuschimi, pticami). Raspredelenie genotipov v gruppah shtammov ot iksodovyh kleschey i pozvonochnyh zhivotnyh razlichalos'. Shtammy VKE genotipov 1 i 2 dostoverno chasche vydelyalis' ot gryzunov, genotipa 3 - ot iksodovyh kleschey (p˂0,05). V gruppe shtammov, izolirovannyh ot iksodovyh kleschey preobladali izolyaty genotipa 3 (85,5%), iz kotoryh 29,6% sostavili shtammy subgenotipa «Vasil'chenko», 25,6% - subgenotipa «Zausaev». Genotipy 1 i 2 byli predstavleny edinichnymi izolyatami (3,3% i 1,3% sootvetstvenno). Sredi shtammov, izolirovannyh ot teplokrovnyh hozyaev, dolya genotipa 1 sostavila 35,3%, genotipa 2 –11,8%, genotipa 3 - 35,3%. Bolee poloviny shtammov genotipa 3 (58,3%) byli otneseny k subgenotipu «Vasil'chenko», shtammov subgenotipa «Zausaev» ne vyyavleno. Poluchennye dannye pozvolyayut predpolozhit', chto iksodovye kleschi yavlyayutsya amplifikatorami VKE genotipa 3, a teplokrovnye zhivotnye – genotipa 1. Vyyavleny opredelennye razlichiya fenotipicheskih priznakov shtammov, izolirovannyh ot kleschey i teplokrovnyh zhivotnyh. Ustanovleno, chto shtammy, izolirovannye ot kleschey, bolee odnorodny po svoey antigennoy harakteristike, menee geterogenny po S-priznaku, chem shtammy, vydelennye ot teplokrovnyh istochnikov. Shtammy, vydelennye ot teplokrovnyh proyavlyayut neskol'ko bol'shuyu ustoychivost' k progrevaniyu pri odnovremennom snizhenii reprodukcii virusa pri temperature 42°S, obladayut bol'shey virulentnost'yu po sravneniyu so shtammami, vydelennymi ot kleschey.
Keywords:
virus kleschevogo encefalita, genotip, geneticheskie svoystva, fenotipicheskie svoystva, geneticheskie markery, istochnik izolyacii, Vostochnaya Sibir'
virus kleschevogo encefalita, genotip, geneticheskie svoystva, fenotipicheskie svoystva, geneticheskie markery, istochnik izolyacii, Vostochnaya Sibir'
1. Spislit stat'ya po istochnikam izolyacii
