Sankt-Peterburg, St. Petersburg, Russian Federation
Sankt-Peterburg, St. Petersburg, Russian Federation
Sankt-Peterburg, St. Petersburg, Russian Federation
Sankt-Peterburg, St. Petersburg, Russian Federation
Moskva, Moscow, Russian Federation
Sankt-Peterburg, St. Petersburg, Russian Federation
Sankt-Peterburg, St. Petersburg, Russian Federation
HOCl obrazuetsya pri stimulyacii neytrofilov blagodarya aktivnosti mieloperoksidazy. Dlya selektivnoy spektrofotometricheskoy detekcii HOCl predlozhen celestinovyy siniy B (CB). Cel'yu dannoy raboty yavilas' razrabotka fluorescentnyh metodov ocenki produkcii HOCl neytrofilami. Produkt reakcii CB s HOCl fluoresciroval pri 578 nm (vozbuzhdenie 487 nm). Metodom konfokal'noy mikroskopii poluchili izobrazheniya aktivirovannyh neytrofilov, okrashennyh CB. Pri protochnoy citometrii fluorescenciya aktivirovannyh 50 nM PMA neytrofilov byla v 5 raz vyshe (p < 0,05), chem u kletok bez aktivatora.
HOCl, hlornovatistaya kislota, mieloperoksidaza, celestinovyy siniy B, neytrofily, konfokal'naya mikroskopiya, protochnaya citometriya, fluorescenciya
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