Voronezh, Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
The object of the research were 28 plants with valuable breeding genotypes of the initial 40-year-old variety-testing cultures of white poplar (Populus alba L.), gray poplar (P. сanescens Sm.), presented by variety of samples of different origins. The purpose of the research was the selection of the most effective microsatellite primers for genetic identification of valuable breeding genotypes of poplar. The selection of high-polymorphic markers was conducted by testing a group of 12 SSR primers.
white poplar, gray poplar, genetic polymorphism, genetic distances, SSR-marker, sertification
1. Kagan D.I., Kovalevich O.A., Matveenko A.V. Populyatsionno-geneticheskiy analiz nasazhdeniy duba chereshchatogo (Querius robur. L.) yuga Belarusi na osnove ispol'zovaniya RAPD-markerov [Population-genetic analysis of pedunculate oak (Querius robur. L.) stands in the south of Belarus on the basis of using RAPD-markers]. Sovremennoe sostoyanie, problemy i perspektivy lesovosstanovleniya i lesorazvedeniya na genetiko-selektsionnoy osnove: Materialy mezhdunarod. nauchno-praktich. konf., 10 sentyabrya 2009 g., Gomel’ [Current state, problems and perspectives of reforestation and afforestation in the genetic-selection basis: Proceedings of the International Scientific-practical. Conf. on September 10, 2009, Gomel]. Gomel, 2009, pp. 59-63. (In Russian)
2. Padutov V.E., Baranov O.Iu., Kagan D.I., Kovalevich O.A., Ostrikova M.Ya., Panteleev S.V., Ivanovskaya S.I., Kulagin D.V. Molekulyarnaya genetika v praktike lesnogo khozyaystva Belarusi [Molecular genetics in the practice of Belarus forestry]. Molekulyarnaya genetika v praktike lesnogo khozyaystva: sostoyanie, problemy i perspektivy primeneniya: Sb. dokladov pervogo Mezhdunarod. soveshchaniya-seminara Moskva, 28-29 maya 2014 goda [Molecular genetics in forestry practice: state, problems and perspectives of application: Coll. of reports of the first international meeting-seminar, Moscow, May 28-29, 2014]. Moscow, 2014, pp. 4-7. (In Russian)
3. Svetlakova T.N., Boboshina I.V., Nechaeva Iu.S., Boronnikova S.V. Geneticheskaya differentsiatsiya populyatsiy Populus tremula v Permskom krae na osnove ISSR - markerov [Genetic differentiation of Populus tremula populations in the Perm region, based on ISSR - markers]. Agrarnyy vestnik Urala [Agricultural Gazette of Ural], 2012, no.11, pp. 11-13. (In Russian)
4. Sivolapov A.I. Topol' sereyushchiy, genetika, selektsiya, razmnozhenie [Poplar graying, it’s genetics, selection, reproduction]. Voronezh, 2005, 157 p. (In Russian)
5. Tsarev A.P. Sortovedenie topolya [Varietology of poplar]. Voronezh, 1985, 152 p. (In Russian)
6. Hussein A.S. Nalbandyan A.A., Fedulova T.P., Bogacheva N.N. Efficient and nontoxic DNA isolation method for PCR analysis. Russian Agricultural Sciences, 2014, Vol. 40, Issue 3, pp. 177-178.
7. Monclus R., Leplé J.-Ch., Bastien C., Bert P.-F., Villar M., Marron N., Brignolas F., Jorge V. Integrating genome annotation and QTL position to identify candidate genes for productivity, architecture and water-use efficiency in Populus spp. Moncluset al. BMC Plant Biology, 2012, no. 12, p. 173.
8. Santos-del-Blanco L., de-Lucas A.I., Sierra R., Hidalgo E. Caracterización, mediante microsatélites nucleares, de Populus x canescens (Aiton) Sm. en la Cuenca del Duero (Castilla y León). 5 Congreso Forestal Español. Montes y sociedad: saber qué hacer, 2009, pp. 2-9.
9. Santos-del-Blanco L., de-Lucas A. I., González-Martínez S.C., Sierra-de-Grado R., Hidalgo E. Extensive Clonal Assemblies in Populus alba and Populus x canescens from the Iberian Peninsula. Tree Genetics & Genomes, 2012, no. 9, pp. 499-510.
10. Schoot J. van der., Pospísˇková M., Vosman B., Smulders M.J.M. Development and characterization of microsatellite markers in black poplar (Populus nigra L.). Theor. Appl. Genet., 2000, no.101, pp. 317-322.
11. Smulders M. J. M., Beringen R., Volosyanchuk R., Broeck A.V., Schoot J. van der, Arens P., Vosman B. Natural hybridisation between Populus nigra L. and P. x canadensis Moench. Hybrid offspring competes for niches along the Rhine river in the Netherlands. Tree Genetics & Genomes, 2008, no.4, pp. 663-675.
12. Vendramin G.G., Hansen O.K. Molecular markers for characterizing diversity in forest trees. Conservation and management of forest genetic resources in Europe. Zvolen, 2005, pp. 337-368.
