Objective of research: to estimate the efficacy of fractionated antigen of mature cattle parasites Setaria labiato-papillosa in diagnosisof dirofilariasis (Dirofilaria immitis). Materials and methods: Antigens of mature Setaria labiato-papillosa, reference sera frominfected and not infected dogs. Reaction of immunodiffusion (RID) in agar gel using reference sera from dogs infected with D. immitis, and extracts from S. labiato-papillosa and D. immitis. Gel-chromatographic method on Pharmacia columns of Superose 12 Prep Grade and immunoenzymatic reaction (IER). Results and discussion: The reaction of immunodiffusion (RID) was used to detectcrossreacting antigens of D. immitis and S. labiato-papillosa. The fractionation of antigen-extract from S. labiato-papillosa allows to get 40 protein fractions, 7 (17,5%) of which were of interest for diagnosis of dirofilariasis. The evaluation of these fractions with respect to diagnosis of dirofilariasis was performed by immunoenzymatic reaction with 47 serum samples from dogs including 11 serum samples from D. immitis infected dogs; 6 — D. repens; 3 — Babesia canis; 3 — Toxocara canis; 9 — samples from dogs with non-parasitic pathology and 15 — from clinically healthy dogs.These results revealed that in one of 11 serum samples infected by D. immitis, and in two of 6 serum samples infected by D. repens — a false-negative response has been registered. The test sensitivity was 82,4%, the specificity — 83,3%. The sensitivity of the similar test with these sera using the purified antigen extract from mature D. immitis, was also 82,4%, and the specificity — 86,7%. The comparability of diagnostic test results for dirofilariasis by IER using antigenic preparations from Setaria and Dirofilaria allows to come to a conclusion that it is possible to use S. labiatopapillosa as a source of antigen for diagnosis of dirofilariasis (D. immitis).
Setaria labiato-papillosa, Dirofilaria immitis, cattle, dog sera, fractionated antigen, immunoenzymatic reaction.
Введение
За последние годы произошло значительное расширение ареала распространения дирофилярий, что привело к росту заболеваемости человека и животных дирофиляриозом и явилось следствием неослабевающего научного интереса к изучению этой медико- ветеринарной проблемы [6-10, 12, 19, 20].
Прижизненная диагностика дирофиляриоза, возбудителями которой являются D. immitis и D. repens, как правило, базируется на обнаружении микрофилярий в крови и их идентификации. Но поскольку дирофиляриоз продолжительное время может протекать бессимптомно, особенно при низкой интенсивности инвазии и соответственно низкой микрофиляриемии, то эти методы фактически не дают должного эффекта.
В таких случаях особый смысл приобретают исследования по разработке и использованию иммунодиагностических тестов, основу которых составляет выявление циркулирующих антигенов или антител, синтезируемых в организме инвазированного животного или человека.
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