Foods and Raw Materials Foods and Raw Materials Foods and Raw Materials 2308-4057 2310-9599 56973 10.21603/2308-4057-2023-1-558 Research Article Research Article Research Article Liquid products of meat and bone meal pyrolysis: comprehensive assessment by chromatographic methods Liquid products of meat and bone meal pyrolysis: comprehensive assessment by chromatographic methods https://orcid.org/0000-0002-9175-2050 Logutov Vladimir I. Logutov Vladimir I. https://orcid.org/0000-0003-4431-1727 Abdrakhmanov Valery I. Abdrakhmanov Valery I. https://orcid.org/0000-0002-9140-5390 Kulikovskii Andrey V. Kulikovskii Andrey V. a.kulikovskii@fncps.ru https://orcid.org/0000-0002-3754-0938 Knyazeva Alexsandra S. Knyazeva Alexsandra S. https://orcid.org/0000-0001-7693-3032 Utiyanov Dmitry A. Utiyanov Dmitry A. Dzerzhinsk branch of the Lobachevsky State University of Nizhny Novgorod Dzerzhinsk Россия Dzerzhinsk branch of the Lobachevsky State University of Nizhny Novgorod Dzerzhinsk Russian Federation LLC Areal Medical Moscow Россия LLC Areal Medical Moscow Russian Federation V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences Moscow Россия V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences Moscow Russian Federation V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences Moscow Россия V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences Moscow Russian Federation V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences Moscow Россия V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences Moscow Russian Federation 17 03 2023 17 03 2023 11 1 94 105 03 02 2022 03 05 2022 https://jfrm.ru/en/issues/20672/21269/

Dorogov’s antiseptic stimulators (fractions 2 and 3) are products of meat and bone meal pyrolysis that are used to treat farm animals. However, there is a lack of detailed information about their chemical composition. We aimed to study individual compositions of organic substances in the water- and oil-soluble condensates of these preparations. Dorogov’s antiseptic stimulators ASD-2F and ASD-3F (Agrovetzashchita, Russia) were used as samples of the water- and oil-soluble condensates of meat and bone meal pyrolysis. Volatile substances were identified by gas chromatography and gas chromatography-mass spectrometry, while amino acids were determined by high-performance liquid chromatography. The initial water-soluble condensate contained ammonium salts, amides of carboxylic acids, N-heterocyclic compounds, hydantoins, amino acids, and dipeptides, with a total content of 8% of the condensate’s weight. Its dehydrated concentrate had almost no ammonium salts and amides of carboxylic acids, but its contents of hydantoins, amino acids, dipeptides, and lowvolatile nitrogen-containing heterocycles were 10–15 times as high as those in the initial condensate. The condensate contained 13 dipeptides and 19 amino acids with a total content of 2.5%. According to gas chromatography-mass spectrometry, the oilsoluble condensate contained over 30% of nitriles; 7–10% of higher and aromatic hydrocarbons, phenols, and amides (with esters); and 1–3% of N-heterocyclic compounds, naphthalenes, pyridines, and dipeptides. The nitrogen-containing heterocycles, as well as dipeptides, were similar to those in the water-soluble condensate. We identified 80% of individual organic substances in the water-soluble pyrolytic condensate. Together with its concentrate, they contained more than 220 organic substances divided into 10 main groups. The oil-soluble condensate consisted of over 350 individual organic compounds. The full composition of the preparations can be further identified by three-quadrupole liquid mass spectrometry.

Dorogov’s antiseptic stimulators (fractions 2 and 3) are products of meat and bone meal pyrolysis that are used to treat farm animals. However, there is a lack of detailed information about their chemical composition. We aimed to study individual compositions of organic substances in the water- and oil-soluble condensates of these preparations. Dorogov’s antiseptic stimulators ASD-2F and ASD-3F (Agrovetzashchita, Russia) were used as samples of the water- and oil-soluble condensates of meat and bone meal pyrolysis. Volatile substances were identified by gas chromatography and gas chromatography-mass spectrometry, while amino acids were determined by high-performance liquid chromatography. The initial water-soluble condensate contained ammonium salts, amides of carboxylic acids, N-heterocyclic compounds, hydantoins, amino acids, and dipeptides, with a total content of 8% of the condensate’s weight. Its dehydrated concentrate had almost no ammonium salts and amides of carboxylic acids, but its contents of hydantoins, amino acids, dipeptides, and lowvolatile nitrogen-containing heterocycles were 10–15 times as high as those in the initial condensate. The condensate contained 13 dipeptides and 19 amino acids with a total content of 2.5%. According to gas chromatography-mass spectrometry, the oilsoluble condensate contained over 30% of nitriles; 7–10% of higher and aromatic hydrocarbons, phenols, and amides (with esters); and 1–3% of N-heterocyclic compounds, naphthalenes, pyridines, and dipeptides. The nitrogen-containing heterocycles, as well as dipeptides, were similar to those in the water-soluble condensate. We identified 80% of individual organic substances in the water-soluble pyrolytic condensate. Together with its concentrate, they contained more than 220 organic substances divided into 10 main groups. The oil-soluble condensate consisted of over 350 individual organic compounds. The full composition of the preparations can be further identified by three-quadrupole liquid mass spectrometry.

 Pyrolysis chemical composition water-soluble condensate oil-soluble condensate meat and bone meal highperformance liquid chromatography gas chromatography gas chromatography-mass spectrometry triple quadrupole liquid mass spectrometry Pyrolysis chemical composition water-soluble condensate oil-soluble condensate meat and bone meal highperformance liquid chromatography gas chromatography gas chromatography-mass spectrometry triple quadrupole liquid mass spectrometry The study was part of the state assignment to V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences (FNEN-2019-0009). The study was part of the state assignment to V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences (FNEN-2019-0009).

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