The article discusses topical issues of the application of labor regulation in modern organizations. It is noted that the system of labor regulation, as if by invisible threads, creates harmony in the organization’s management system and contributes to the formation of business sustainability. The content and direction of regulation of labor activity are considered. The authors of the article convincingly prove that the analysis and optimization of the existing set of regulations used in the personnel management process allows the organization to ensure more effective personnel management, improve productivity and efficiency, as well as reduce the risks associated with the incorrect application of certain documents and procedures. From a practical point of view, the results of the authors’ study of the main problems of labor regulation in domestic production organizations and detailed recommendations for their elimination and the direct development of the regulatory system are of interest.