SPECIALIZED SOFTWARE FOR ANALYSIS OF OPTICAL DENSITY DIGITAL IMAGES MICROPREPARATIONS
Rubrics: EDITORIAL PORTOLIO
Authors:
Type:
Article
Pages:
from 0
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Status:
Published
Received:
26.12.2014
Accepted:
26.12.2014
Published:
26.12.2014
Language:
Russian
Keywords:
microdensitometry, digital photography, histochemistry.
Abstract (English):
The development of software for densitometric analysis is actual nowadays. The main task in performing the densitometric analysis is the estimation of light quantity rate which passed through the product of histochemical reaction of examined object to some sample transparent field without an analyzed object, delaying the light flow, such as slide-glass, coverslip, mounts environment. As a a rule, the information on the color of reaction products is of no interest to the study. That is why monochrome digital cameras are preferred for densitometric analysis. The article presents the issues of creating software for the analysis of digital micrographs of biological objects. The introduction sets out the problem statement and artifacts that result in errors during the densitometric analysis. The problem of constructing software for digital image processing micropreparations obtained using a microscope. The realization of software, mathematical methods of treatment, the results of applying these methods to real data.
The development of software for densitometric analysis is actual nowadays. The main task in performing the densitometric analysis is the estimation of light quantity rate which passed through the product of histochemical reaction of examined object to some sample transparent field without an analyzed object, delaying the light flow, such as slide-glass, coverslip, mounts environment. As a a rule, the information on the color of reaction products is of no interest to the study. That is why monochrome digital cameras are preferred for densitometric analysis. The article presents the issues of creating software for the analysis of digital micrographs of biological objects. The introduction sets out the problem statement and artifacts that result in errors during the densitometric analysis. The problem of constructing software for digital image processing micropreparations obtained using a microscope. The realization of software, mathematical methods of treatment, the results of applying these methods to real data.
Keywords:
microdensitometry, digital photography, histochemistry.
microdensitometry, digital photography, histochemistry.
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