This paper presents the study of biological effect of drugs of acid-soluble low-molecular non-collagenous proteins of bone tissue of bovine at different stages of shin fracture union in laboratory mice. Preparations of low- molecular bone tissue proteins were received from tissue of a femur of a bovine by consecutive application of acid demineralization, a dialysis and a high performance liquid chromatography. Using this method, two preparations of bone proteins with a molecular weight 6,5 and 2,1 kDa were received, which biological effect was investigated by intraperitoneal injection in laboratory mice with a shin fracture. After 3, 7 and 30th days, the changes of biochemical markers of blood serum and mineral components of a bone were investigated. In blood serum the authors investigated content of inorganic phosphate, the general calcium, the general protein, activity of the alkaline phosphatase and acid phosphatase. In bone tissue, the content of water, phosphate and calcium was studied. Effects of bone tissue preparations shown decreasing activity of acid phosphatase, decrease in the content of inorganic phosphate and calcium ions in blood serum at early stages of fracture union.
low-molecular non-collagenous proteins of bone tissue, high performance liquid chromatography, osteogenesis.
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