EXPRESSION OF COLLAGENS IN THE DAMAGE AREA AT ABDOMINAL ADHESIONS
Rubrics: SURGERY & NEUROSURGERY
Authors:
1.
Irkutsk Scientific Center of Surgery and Traumatology
(Deputy Director for Science)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
2.
Irkutsk Scientific Center of Surgery and Traumatology
(Scientific Laboratory DepartmentHead)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
3.
Irkutsk Scientific Center of Surgery and Traumatology
(Laboratory of cell Pathophysiology and Biochemistry, Head)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
4.
Irkutsk State Medical University
(Assistant Professor at the Department of Hospital Surgery)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
5.
Irkutsk Scientific Center of Surgery and Traumatology
(Unit of Purulent Surgery, Surgeon)
Irkutsk, Irkutsk region, Russian Federation
Irkutsk, Irkutsk region, Russian Federation
Type:
Article
Pages:
from 172
to 176
Status:
Published
Received:
14.11.2017
Accepted:
30.11.2017
Published:
30.11.2017
Subject area:
UDK 61 Медицина. Охрана здоровья
Language:
Russian
Keywords:
spaechnaya bolezn', kollagen, kollagen Va3, modelirovanie
Abstract (English):
Posleoperacionnaya spaechnaya bolezn' yavlyaetsya ser'eznoy problemoy v hirurgii, odnako na nastoyaschiy mo- ment molekulyarnye mehanizmy razvitiya spaechnogo processa ostayutsya nedostatochno izuchennymi. Cel' issledovaniya: izuchit' dinamiku ekspressii genov, kodiruyuschih sintez kollagena, pri povrezhdenii seroznoy obolochki na primere bryushiny v usloviyah asepticheskogo vospaleniya. V eksperimente modelirovali asepticheskiy vospalitel'nyy process v bryushnoy polosti, izuchali mikro- i makroskopicheskuyu kartinu zony povrezhdeniya, osuschestvlyali immunoflyuorescentnoe okrashivanie na kollagen I tipa. Ustanovleno, chto process spaykoobrazovaniya pri povrezhdenii bryushiny v asepticheskih usloviyah dostigaet maksimuma k 30-m sutkam nablyudeniya. V etot zhe srok otmechaetsya i maksimum sinte- za kollagena v fibroblastah v zone reparacii, chto otmecheno pri immunoflyuorescentnom issledovanii. Vpervye vyyavleno aktivnoe vovlechenie kollagena tipa V al'fa-3 v process spaykoobrazovaniya: otmecheno kak rannee (s 1-h sutok), tak i maksimal'no intensivnoe (kratnost' povysheniya do 166,96 raza po sravne- niyu s intaktnymi zhivotnymi) ego povyshenie. Vozmozhno, vyyavlennaya nami giperekspressiya kollagena V al'fa-3 yavlyaetsya vazhnym zvenom v patogeneze spaykoobrazovaniya v bryushnoy polosti.
Posleoperacionnaya spaechnaya bolezn' yavlyaetsya ser'eznoy problemoy v hirurgii, odnako na nastoyaschiy mo- ment molekulyarnye mehanizmy razvitiya spaechnogo processa ostayutsya nedostatochno izuchennymi. Cel' issledovaniya: izuchit' dinamiku ekspressii genov, kodiruyuschih sintez kollagena, pri povrezhdenii seroznoy obolochki na primere bryushiny v usloviyah asepticheskogo vospaleniya. V eksperimente modelirovali asepticheskiy vospalitel'nyy process v bryushnoy polosti, izuchali mikro- i makroskopicheskuyu kartinu zony povrezhdeniya, osuschestvlyali immunoflyuorescentnoe okrashivanie na kollagen I tipa. Ustanovleno, chto process spaykoobrazovaniya pri povrezhdenii bryushiny v asepticheskih usloviyah dostigaet maksimuma k 30-m sutkam nablyudeniya. V etot zhe srok otmechaetsya i maksimum sinte- za kollagena v fibroblastah v zone reparacii, chto otmecheno pri immunoflyuorescentnom issledovanii. Vpervye vyyavleno aktivnoe vovlechenie kollagena tipa V al'fa-3 v process spaykoobrazovaniya: otmecheno kak rannee (s 1-h sutok), tak i maksimal'no intensivnoe (kratnost' povysheniya do 166,96 raza po sravne- niyu s intaktnymi zhivotnymi) ego povyshenie. Vozmozhno, vyyavlennaya nami giperekspressiya kollagena V al'fa-3 yavlyaetsya vazhnym zvenom v patogeneze spaykoobrazovaniya v bryushnoy polosti.
Keywords:
spaechnaya bolezn', kollagen, kollagen Va3, modelirovanie
spaechnaya bolezn', kollagen, kollagen Va3, modelirovanie
1. Ekspressiya kollagenov v zone povrezhdeniya
