Acta biomedica scientifica

2541-9420 2587-9596

14509

10.12737/23404

Экспериментальные исследования в биологии и медицине

Experimental Study in biology and medicine

Экспериментальные исследования в биологии и медицине

expressIon of molecular lymphatIc markers In human choroId

Экспрессия молекулярных лимфатических маркеров сосудистой оболочке глаза человека

Ноговицина

Сабина Робертовна

Nogovitsina

Sabina Робертовна

nogovitsina.niikel@gmail.com

10 12 2016

1 5 114 116

https://naukaru.ru/en/nauka/article/14509/view

Целью работы было выявление лимфатических путей оттока в сосудистой оболочке глаза человека. Проведено иммуногистохимическое исследование хориоидеи с использованием антител к специфическим лимфатическим и кровеносным эндотелиальным маркерам. Выявлены Prox-1+-, LYVE-1+-, и Podopla-nin+- каналы и лакуны, ограниченные эндотелиоподобными клетками и фибробластами. Положительное окрашивание на маркеры лимфатических сосудов и отсутствие их окрашивания на маркер кровеносных сосудов CD34 дает основание считать выявленные структуры лимфатическими.

The purpose of this study was to research lymphatic outflow tract in the human choroid. Using a light microscopy and immunohistochemistry, structure of the choroid was investigated. We used antibodies against lymphaticendotheli-al-specificmarkers Prox-1, LYVE-1, Podoplanin, endothelial blood vessels marker CD34 and fibroblasts growth factor receptor FGFR. The choroid was found to contain lymphatic canals in choroidal stroma and a layer of choriocapillaris, and lymphatic lacunae in the suprachoroid lamina, limited endothelium-like cells, positively stained for markers of lymphatic vessels Prox-1, LYVE-1, Podoplanin, and fibroblasts and pigment cells. These endothelium-like cells were shown positive Prox-1, LYVE-1 and Podoplanin staining and negative CD34 staining; therefore we consider that these canals and lacunae are lymphatic structures.

Структурая оболочка глаза лимфатические каналы и лакуны

choroid lymphatic lacunae lymphatic channel

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